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Servicebio Inc
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Fisher Scientific
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Nanjing Jiancheng Bioengineering Research Institute Co Ltd
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Beyotime
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Journal: Frontiers in Pharmacology
Article Title: Modified gegen qinlian decoction ameliorates Veillonella parvula -exacerbated ulcerative colitis via restoration of intestinal mucosal barrier function
doi: 10.3389/fphar.2026.1803816
Figure Lengend Snippet: V. parvula exacerbates colonic mucus barrier damage in DSS-induced colitis mice, while the MGQD ameliorates this V. parvula -exacerbated damage. (A,B) AB-PAS staining in mouse colon. (C) The IF staining of mouse colon for Muc2 (red) and DAPI (blue). Data are expressed as mean ± S.D. (n = 6). #P < 0.05, ##P < 0.01, ###P < 0.001 vs. the DSV group. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the DSS group.
Article Snippet: Hematoxylin and eosin (H&E) and
Techniques: Staining
Journal: Frontiers in Pharmacology
Article Title: Modified gegen qinlian decoction ameliorates Veillonella parvula -exacerbated ulcerative colitis via restoration of intestinal mucosal barrier function
doi: 10.3389/fphar.2026.1803816
Figure Lengend Snippet: V. parvula exacerbates colonic barrier damage in DSS-induced colitis pseudo-germ-free mice, while the MGQD ameliorates this V. parvula -exacerbated damage. (A) The AB-PAS staining in mouse colon. (B) The IF staining of mouse colon for Muc2 (red) and DAPI (blue). (C) The Western blot of mouse colon. The data are expressed as the means ± S.D. (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001 vs. the VD group.
Article Snippet: Hematoxylin and eosin (H&E) and
Techniques: Staining, Western Blot
Journal: STAR Protocols
Article Title: Protocol for developing a mucus-producing gut-on-a-chip model from Caco-2 and HT29-MTX-E12 cells
doi: 10.1016/j.xpro.2025.104321
Figure Lengend Snippet: Mucus production, barrier integrity and cell viability evaluation for GoC characterization (A) Mucus production in co-culture 9:1 and 7:3 with Alcian blue/Periodic Acid Schiff’s staining. (B) Barrier integrity with LY (20 μg/ml) for co-culture 9:1 and 7:3 day 2, 4, 6, 8; blank - empty chip (Data are represented as mean ± SD. Test: One-way ANOVA t test, p < 0.01 “∗∗”, p < 0.0001 “∗∗∗∗”, n = 4). (C) Barrier integrity with LY (20 μg/ml) for co-culture 9:1 and 7:3 day 2, 4, 6, 8 (Data are represented as mean ± SD. Test: Two-way ANOVA t test, p < 0.05 “∗”, non-significant “ns”, n = 4). (D) Cell viability with CCK-8 cell viability kit for co-culture 9:1, 7:3 and viability control for co-culture 7:3 (0.25% Triton X-100 added in CM in flow for 24 hours at 2 μL/min) (Data are represented as mean ± SD. Test: One-way ANOVA t test, non-significant “ns”, p < 0.0001 “∗∗∗∗”, n = 4). (E) Variance using barrier integrity test with LY (20 μg/ml) for co-culture 7:3 day 2, 4, 6, 8 (Data are represented as mean ± SD. Test: Two-way ANOVA t test, p < 0.05 “∗”, p < 0.0001 “∗∗∗∗”, non-significant “ns”, n = 12–3 independent chips). (F) Variance using cell viability test with CCK-8 cell viability kit for co-culture 7:3 on day 8 in three chips independently No1, No2, No3 (n = 4) and all of them combined No1 + No2 + No3 (n = 12) (Data are represented as mean ± SD. Test: One-way ANOVA t test, non-significant “ns”).
Article Snippet:
Techniques: Co-Culture Assay, Staining, CCK-8 Assay, Control